Quantitation of prenylcysteines by a selective cleavage reaction.
The allylic thioether bond of the prenylcysteines of prenylated proteins has been shown to be cleaved by 2-naphthol under alkaline conditions to yield substituted naphthopyrans. These products are readily resolved from interfering materials by HPLC and have a strongly absorbing chromophore. Thus, th...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1991
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1946384/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.21.9668 |
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