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Direct selection: a method for the isolation of cDNAs encoded by large genomic regions.

We have developed a strategy for the rapid enrichment and identification of cDNAs encoded by large genomic regions. The basis of this "direct selection" scheme is the hybridization of an entire library of cDNAs to an immobilized genomic clone. Nonspecific hybrids are eliminated and selected cDNAs ar...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Lovett, M, Kere, J, Hinton, L M
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52771/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1946378/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.21.9628
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