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Combining multi-mutant and modular thermodynamic cycles to measure energetic coupling networks in enzyme catalysis
We measured and cross-validated the energetics of networks in Bacillus stearothermophilus Tryptophanyl-tRNA synthetase (TrpRS) using both multi-mutant and modular thermodynamic cycles. Multi-dimensional combinatorial mutagenesis showed that four side chains from this “molecular switch” move coordina...
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| Udgivet i: | Struct Dyn |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Crystallographic Association
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5272822/ https://ncbi.nlm.nih.gov/pubmed/28191480 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4974218 |
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