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Combining multi-mutant and modular thermodynamic cycles to measure energetic coupling networks in enzyme catalysis

We measured and cross-validated the energetics of networks in Bacillus stearothermophilus Tryptophanyl-tRNA synthetase (TrpRS) using both multi-mutant and modular thermodynamic cycles. Multi-dimensional combinatorial mutagenesis showed that four side chains from this “molecular switch” move coordina...

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Bibliografiske detaljer
Udgivet i:Struct Dyn
Main Authors: Carter, Charles W., Chandrasekaran, Srinivas Niranj, Weinreb, Violetta, Li, Li, Williams, Tishan
Format: Artigo
Sprog:Inglês
Udgivet: American Crystallographic Association 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5272822/
https://ncbi.nlm.nih.gov/pubmed/28191480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4974218
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