Combining multi-mutant and modular thermodynamic cycles to measure energetic coupling networks in enzyme catalysis

We measured and cross-validated the energetics of networks in Bacillus stearothermophilus Tryptophanyl-tRNA synthetase (TrpRS) using both multi-mutant and modular thermodynamic cycles. Multi-dimensional combinatorial mutagenesis showed that four side chains from this “molecular switch” move coordina...

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Vydáno v:Struct Dyn
Hlavní autoři: Carter, Charles W., Chandrasekaran, Srinivas Niranj, Weinreb, Violetta, Li, Li, Williams, Tishan
Médium: Artigo
Jazyk:Inglês
Vydáno: American Crystallographic Association 2017
Témata:
Transactions from the 66th Annual Meeting of the American Crystallographic Association (Aca)
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5272822/
https://ncbi.nlm.nih.gov/pubmed/28191480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4974218
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