Thermostabilisation of membrane proteins for structural studies

The thermostability of an integral membrane protein in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals suitable for structure determination. However, many mammalian membrane proteins are too unstable for crystallisation. We developed a thermo...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Magnani, Francesca, Serrano-Vega, Maria J., Shibata, Yoko, Abdul-Hussein, Saba, Lebon, Guillaume, Miller-Gallacher, Jennifer, Singhal, Ankita, Strege, Annette, Thomas, Jennifer A., Tate, Christopher G.
Format: Artigo
Language:Inglês
Published: 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5268090/
https://ncbi.nlm.nih.gov/pubmed/27466713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.088
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