Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay

Ongoing evolution of viral pathogens is a significant issue in diagnostic virology employing TaqMan qPCR/RT-qPCR. Specific concerns are related to false negativity due to probe binding failure. One option for compensating for such deficiency is to integrate a second identically labelled probe in the...

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Udgivet i:Sci Rep
Main Authors: Nagy, Alexander, Vitásková, Eliška, Černíková, Lenka, Křivda, Vlastimil, Jiřincová, Helena, Sedlák, Kamil, Horníčková, Jitka, Havlíčková, Martina
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5264587/
https://ncbi.nlm.nih.gov/pubmed/28120891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep41392
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