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Phage display of ricin B chain and its single binding domains: system for screening galactose-binding mutants.

We demonstrate that the B chain of ricin toxin preserves its lectin activity when expressed as a fusion protein on the surface of fd phage. Moreover, B chain, which folds into two topologically similar globular domains, can be dissected into amino-terminal and carboxyl-terminal domains to form singl...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Swimmer, C, Lehar, S M, McCafferty, J, Chiswell, D J, Blättler, W A, Guild, B C
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC525569/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1373889/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.9.3756
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