Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active sc...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Monedero, Vicente, Rodríguez-Díaz, Jesús, Viana, Rosa, Buesa, Javier, Pérez-Martínez, Gaspar
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2004
Assuntos:	Physiology and Biotechnology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC525132/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15528568/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.11.6936-6939.2004
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