Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active sc...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Monedero, Vicente, Rodríguez-Díaz, Jesús, Viana, Rosa, Buesa, Javier, Pérez-Martínez, Gaspar
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2004
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC525132/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15528568/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.11.6936-6939.2004
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