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Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase

Unveiling the determinants for transferase and hydrolase activity in glycoside hydrolases would allow using their vast diversity for creating novel transglycosylases, thereby unlocking an extensive toolbox for carbohydrate chemists. Three different amino acid substitutions at position 220 of a GH1 β...

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Pubblicato in:Appl Microbiol Biotechnol
Autori principali: Lundemo, Pontus, Karlsson, Eva Nordberg, Adlercreutz, Patrick
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Berlin Heidelberg 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5247548/
https://ncbi.nlm.nih.gov/pubmed/27678115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-016-7833-9
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