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Metal ion-mediated polymer superquenching for highly sensitive detection of kinase and phosphatase activities

An assay technology for high-throughput screening of kinase and phosphatase activities is introduced. The format is based upon superquenching of fluorescent-conjugated polymers by dye-labeled kinase/phosphatase peptide substrates. The sensor platform is composed of highly fluorescent-conjugated poly...

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Bibliografiske detaljer
Main Authors: Rininsland, Frauke, Xia, Wensheng, Wittenburg, Shannon, Shi, Xiaobo, Stankewicz, Casey, Achyuthan, Komandoor, McBranch, Duncan, Whitten, David
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC524457/
https://ncbi.nlm.nih.gov/pubmed/15494445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0406832101
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