Joining of long double-stranded RNA molecules through controlled overhangs

We describe two methods for creating long (>1 kb) dsRNA molecules with specific, user-controlled overhangs for efficient hybridization and ligation. The two methods create double-stranded RNA (dsRNA) molecules with 5′ overhangs or with 3′ overhangs using T7 RNA polymerase (T7 RNAP) in transcripti...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Dekker, N. H., Abels, J. A., Veenhuizen, P. T. M., Bruinink, M. M., Dekker, C.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2004
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC524314/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15475386/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh138
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