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Padlock oligonucleotides as a tool for labeling superhelical DNA

Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called ‘padlock oligonucleotide’. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-st...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Roulon, Thibaut, Coulaud, Dominique, Delain, Etienne, Le Cam, Eric, Hélène, Claude, Escudé, Christophe
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100311/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809900/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e12
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