Padlock oligonucleotides as a tool for labeling superhelical DNA

Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called ‘padlock oligonucleotide’. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-st...

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Hlavní autoři: Roulon, Thibaut, Coulaud, Dominique, Delain, Etienne, Le Cam, Eric, Hélène, Claude, Escudé, Christophe
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2002
Témata:
NAR Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC100311/
https://ncbi.nlm.nih.gov/pubmed/11809900
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