Padlock oligonucleotides as a tool for labeling superhelical DNA

Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called ‘padlock oligonucleotide’. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-st...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Roulon, Thibaut, Coulaud, Dominique, Delain, Etienne, Le Cam, Eric, Hélène, Claude, Escudé, Christophe
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 2002
Subjects:	NAR Methods Online
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC100311/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11809900/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/30.3.e12
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