Joining of long double-stranded RNA molecules through controlled overhangs

We describe two methods for creating long (>1 kb) dsRNA molecules with specific, user-controlled overhangs for efficient hybridization and ligation. The two methods create double-stranded RNA (dsRNA) molecules with 5′ overhangs or with 3′ overhangs using T7 RNA polymerase (T7 RNAP) in transcription...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Dekker, N. H., Abels, J. A., Veenhuizen, P. T. M., Bruinink, M. M., Dekker, C.
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2004
Matèries:	NAR Methods Online
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC524314/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15475386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh138
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