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Joining of long double-stranded RNA molecules through controlled overhangs
We describe two methods for creating long (>1 kb) dsRNA molecules with specific, user-controlled overhangs for efficient hybridization and ligation. The two methods create double-stranded RNA (dsRNA) molecules with 5′ overhangs or with 3′ overhangs using T7 RNA polymerase (T7 RNAP) in transcripti...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC524314/ https://ncbi.nlm.nih.gov/pubmed/15475386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh138 |
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