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Electrothermal Supercharging of Proteins in Native MS: Effects of Protein Isoelectric Point, Buffer, and nanoESI-Emitter Tip Size

The extent of charging resulting from electrothermal supercharging for protein ions formed from various buffered aqueous solutions using nanoESI emitters with tip diameters between ~1.5 μm and ~310 nm is compared. Charging increases with decreasing tip size for proteins that are positively charged i...

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Bibliographic Details
Published in:Analyst
Main Authors: Mortensen, Daniel N., Williams, Evan R.
Format: Artigo
Language:Inglês
Published: 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5239670/
https://ncbi.nlm.nih.gov/pubmed/27441318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c6an01380e
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