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Electrothermal Supercharging of Proteins in Native Electrospray Ionization

The formation of high charge-state protein ions with nanoelectrospray ionization (nESI) from purely aqueous ammonium bicarbonate solutions at neutral pH, where the proteins have native or native-like conformations prior to ESI droplet formation, is demonstrated. This “electrothermal” supercharging m...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sterling, Harry J., Cassou, Catherine A., Susa, Anna C., Williams, Evan R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3328611/
https://ncbi.nlm.nih.gov/pubmed/22409200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac300468a
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