Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies

BACKGROUND: There is an ever-increasing need of monoclonal antibodies (mAbs) for biomedical applications and fully human binders are particularly desirable due to their reduced immunogenicity in patients. We have applied a strategy for the isolation of antigen-specific B cells using tetramerized pro...

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Bibliografske podrobnosti
izdano v:BMC Biotechnol
Main Authors: Ouisse, Laure-Hélène, Gautreau-Rolland, Laetitia, Devilder, Marie-Claire, Osborn, Michael, Moyon, Melinda, Visentin, Jonathan, Halary, Frank, Bruggemann, Marianne, Buelow, Roland, Anegon, Ignacio, Saulquin, Xavier
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2017
Teme:
Methodology Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5234254/
https://ncbi.nlm.nih.gov/pubmed/28081707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-016-0322-5
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