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Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase chain reaction product detection system to generate a specific detectable signal concomitantly with amplification. An oligonucleotide probe, nonextendable at the 3&...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Holland, P M, Abramson, R D, Watson, R, Gelfand, D H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC52277/
https://ncbi.nlm.nih.gov/pubmed/1871133
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