Enzymatic Analysis of an Amylolytic Enzyme from the Hyperthermophilic Archaeon Pyrococcus furiosus Reveals Its Novel Catalytic Properties as both an α-Amylase and a Cyclodextrin-Hydrolyzing Enzyme

Genomic analysis of the hyperthermophilic archaeon Pyrococcus furiosus revealed the presence of an open reading frame (ORF PF1939) similar to the enzymes in glycoside hydrolase family 13. This amylolytic enzyme, designated PFTA (Pyrococcus furiosus thermostable amylase), was cloned and expressed in...

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Detalhes bibliográficos
Main Authors: Yang, Sung-Jae, Lee, Hee-Seob, Park, Cheon-Seok, Kim, Yong-Ro, Moon, Tae-Wha, Park, Kwan-Hwa
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC522074/
https://ncbi.nlm.nih.gov/pubmed/15466542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.10.5988-5995.2004
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