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Measuring single-molecule nucleic acid dynamics in solution by two-color filtered ratiometric fluorescence correlation spectroscopy

This work presents a general method for determining single-molecule intramolecular dynamics in biomolecules by using a reporter fluorophore, whose fluorescence is quenched or partially quenched as a result of intramolecular motion, and a remote observer fluorophore. These fluorophores were excited i...

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Dades bibliogràfiques
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Li, Haitao, Ren, Xiaojun, Ying, Liming, Balasubramanian, Shankar, Klenerman, David
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC521954/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15452356/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0404295101
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