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Measuring single-molecule nucleic acid dynamics in solution by two-color filtered ratiometric fluorescence correlation spectroscopy

This work presents a general method for determining single-molecule intramolecular dynamics in biomolecules by using a reporter fluorophore, whose fluorescence is quenched or partially quenched as a result of intramolecular motion, and a remote observer fluorophore. These fluorophores were excited i...

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Sonraí Bibleagrafaíochta
Main Authors: Li, Haitao, Ren, Xiaojun, Ying, Liming, Balasubramanian, Shankar, Klenerman, David
Formáid: Artigo
Teanga:Inglês
Foilsithe: National Academy of Sciences 2004
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC521954/
https://ncbi.nlm.nih.gov/pubmed/15452356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404295101
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