Lataa...
Augmenting CRISPR applications in Drosophila with tRNA-flanked Cas9 and Cpf1 sgRNAs
We present tRNA-based vectors for producing multiple clustered regularly-interspaced short palindromic repeats (CRISPR) sgRNAs from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of sgRNAs by processing of flanking tRNAs, permits highly efficient...
Tallennettuna:
| Julkaisussa: | Nat Methods |
|---|---|
| Päätekijät: | , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2016
|
| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5215823/ https://ncbi.nlm.nih.gov/pubmed/27595403 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3972 |
| Tagit: |
Lisää tagi
Ei tageja, Lisää ensimmäinen tagi!
|