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Augmenting CRISPR applications in Drosophila with tRNA-flanked Cas9 and Cpf1 sgRNAs
We present tRNA-based vectors for producing multiple clustered regularly-interspaced short palindromic repeats (CRISPR) sgRNAs from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of sgRNAs by processing of flanking tRNAs, permits highly efficient...
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| Veröffentlicht in: | Nat Methods |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5215823/ https://ncbi.nlm.nih.gov/pubmed/27595403 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3972 |
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