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Microscale Measurements of Michaelis–Menten Constants of Neuraminidase with Nanogel Capillary Electrophoresis for the Determination of the Sialic Acid Linkage

[Image: see text] Phospholipid nanogels enhance the stability and performance of the exoglycosidase enzyme neuraminidase and are used to create a fixed zone of enzyme within a capillary. With nanogels, there is no need to covalently immobilize the enzyme, as it is physically constrained. This enable...

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Bibliographic Details
Published in:Anal Chem
Main Authors: Gattu, Srikanth, Crihfield, Cassandra. L., Holland, Lisa A.
Format: Artigo
Language:Inglês
Published: American Chemical Society 2016
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5214287/
https://ncbi.nlm.nih.gov/pubmed/27936604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b04074
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