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The reliability of Michaelis constants and maximum velocities estimated by using the integrated Michaelis–Menten equation
1. Experimental progress curves were simulated for a reaction obeying Michaelis–Menten kinetics. 2. K(m) and V were estimated (a) by fitting the integrated Michaelis–Menten equation to the progress curves, and (b) from the initial slopes of the curves (i.e. from initial velocities). 3. The integrate...
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| Main Authors: | , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1973
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1165894/ https://ncbi.nlm.nih.gov/pubmed/4778274 |
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