Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.
Reconstitution of the translocation machinery for secretory proteins from purified constituents was performed. SecY was solubilized from SecY/SecE-overproducing Escherichia coli cells and purified by chromatography on ion-exchange and size-exclusion columns. Proteoliposomes active in protein translo...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1991
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52123/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1830665/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.15.6545 |
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