Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

Reconstitution of the translocation machinery for secretory proteins from purified constituents was performed. SecY was solubilized from SecY/SecE-overproducing Escherichia coli cells and purified by chromatography on ion-exchange and size-exclusion columns. Proteoliposomes active in protein translo...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Akimaru, J, Matsuyama, S, Tokuda, H, Mizushima, S
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1991
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52123/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1830665/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.15.6545
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