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Partial Purification of a Megadalton DNA Replication Complex by Free Flow Electrophoresis
We describe a gentle and rapid method to purify the intact multiprotein DNA replication complex using free flow electrophoresis (FFE). In particular, we applied FFE to purify the human cell DNA synthesome, which is a multiprotein complex that is fully competent to carry-out all phases of the DNA rep...
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| Publicado en: | PLoS One |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Public Library of Science
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5201288/ https://ncbi.nlm.nih.gov/pubmed/28036377 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0169259 |
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