Structural and Catalytic Properties of S1 Nuclease from Aspergillus oryzae Responsible for Substrate Recognition, Cleavage, Non–Specificity, and Inhibition

The single–strand–specific S1 nuclease from Aspergillus oryzae is an archetypal enzyme of the S1–P1 family of nucleases with a widespread use for biochemical analyses of nucleic acids. We present the first X–ray structure of this nuclease along with a thorough analysis of the reaction and inhibition...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Kovaľ, Tomáš, Østergaard, Lars H., Lehmbeck, Jan, Nørgaard, Allan, Lipovová, Petra, Dušková, Jarmila, Skálová, Tereza, Trundová, Mária, Kolenko, Petr, Fejfarová, Karla, Stránský, Jan, Švecová, Leona, Hašek, Jindřich, Dohnálek, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5201275/
https://ncbi.nlm.nih.gov/pubmed/28036383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0168832
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