Crystallization of recombinant bifunctional nuclease TBN1 from tomato

The endonuclease TBN1 from Solanum lycopersicum (tomato) was expressed in Nicotiana benthamiana leaves and purified with suitable quality and in suitable quantities for crystallization experiments. Two crystal forms (orthorhombic and rhombohedral) were obtained and X-ray diffraction experiments were...

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Bibliographic Details
Main Authors: Koval’, Tomáš, Lipovová, Petra, Podzimek, Tomáš, Matoušek, Jaroslav, Dušková, Jarmila, Skálová, Tereza, Štěpánková, Andrea, Hašek, Jindřich, Dohnálek, Jan
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2010
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079990/
https://ncbi.nlm.nih.gov/pubmed/21206042
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110048177
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