Proteinase trapping: screening for viral proteinase mutants by alpha complementation.

Many virally encoded proteinases cleave themselves out of a polyprotein, with cleavage occurring usually at their own N terminus. This property was used to develop an in vivo screening system using the lacZ gene fragment of M13mp18. When a fusion protein of the alpha fragment of beta-galactosidase a...

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Bibliographic Details
Main Authors: Liebig, H D, Skern, T, Luderer, M, Sommergruber, W, Blaas, D, Kuechler, E
Format: Artigo
Language:Inglês
Published: 1991
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC52005/
https://ncbi.nlm.nih.gov/pubmed/1648726
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