Proteinase trapping: screening for viral proteinase mutants by alpha complementation.

Many virally encoded proteinases cleave themselves out of a polyprotein, with cleavage occurring usually at their own N terminus. This property was used to develop an in vivo screening system using the lacZ gene fragment of M13mp18. When a fusion protein of the alpha fragment of beta-galactosidase a...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Liebig, H D, Skern, T, Luderer, M, Sommergruber, W, Blaas, D, Kuechler, E
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1991
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52005/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1648726/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.14.5979
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