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Optimisation of the two-dimensional gel electrophoresis protocol using the Taguchi approach
BACKGROUND: Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this approach is the solubilisation of protein mixtures for isoelectric focusing (IEF)...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC517948/ https://ncbi.nlm.nih.gov/pubmed/15357868 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-2-6 |
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