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Enhanced detergent extraction for analysis of membrane proteomes by two-dimensional gel electrophoresis
BACKGROUND: The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocols by varying the detergent composition of the solubilization buffer with a vari...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1184097/ https://ncbi.nlm.nih.gov/pubmed/15941475 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-5956-3-5 |
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