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Use of the U1A protein to facilitate crystallization and structure determination of large RNAs
The preparation of well-ordered crystals of RNAs with complex three-dimensional architecture can be facilitated by engineering a binding site for the spliceosomal protein U1A into a functionally and structurally dispensable stem-loop of the RNA of interest. Once suitable crystals are obtained, the U...
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| Pubblicato in: | Methods Mol Biol |
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| Autore principale: | |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5178131/ https://ncbi.nlm.nih.gov/pubmed/26227038 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2763-0_6 |
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