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Transposon-Mediated Linker Insertion Scanning Mutagenesis of the Escherichia coli McrA Endonuclease

McrA is one of three functions that restrict modified foreign DNA in Escherichia coli K-12, affecting both methylated and hydroxymethylated substrates. We present here the first systematic analysis of the functional organization of McrA by using the GPS-LS insertion scanning system. We collected in-...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Anton, Brian P., Raleigh, Elisabeth A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC516834/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15317774/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.186.17.5699-5707.2004
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