Isolation of temperature-sensitive McrA and McrB mutations and complementation analysis of the McrBC region of Escherichia coli K-12.

We isolated temperature-sensitive mcrA and mcrBC mutants of Escherichia coli. At 42 degrees C, they were unable to restrict the T-even bacteriophages T6gt and T4gt or plasmids encoding cloned DNA methylase genes whose specificities confer sensitivity to the McrA and McrBC nucleases. Complementation...

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Bibliographic Details
Main Authors: Piekarowicz, A, Yuan, R, Stein, D C
Format: Artigo
Language:Inglês
Published: 1991
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC207168/
https://ncbi.nlm.nih.gov/pubmed/1987114
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