Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.

Using the plasmid DNA pSV2-neo (which, when integrated into the cellular genome confers resistance to the antibiotic G418 for selection), we examined and compared the transfection efficiency on NIH 3T3 cells electropermeabilized by applying a sequence of high-frequency unipolar or bipolar square wav...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Tekle, E, Astumian, R D, Chock, P B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2034667/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.10.4230
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