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Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.

Using the plasmid DNA pSV2-neo (which, when integrated into the cellular genome confers resistance to the antibiotic G418 for selection), we examined and compared the transfection efficiency on NIH 3T3 cells electropermeabilized by applying a sequence of high-frequency unipolar or bipolar square wav...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tekle, E, Astumian, R D, Chock, P B
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC51632/
https://ncbi.nlm.nih.gov/pubmed/2034667
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