Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.
Using the plasmid DNA pSV2-neo (which, when integrated into the cellular genome confers resistance to the antibiotic G418 for selection), we examined and compared the transfection efficiency on NIH 3T3 cells electropermeabilized by applying a sequence of high-frequency unipolar or bipolar square wav...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
1991
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2034667/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.10.4230 |
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