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Can terminators be used as insulators into yeast synthetic gene circuits?
BACKGROUND: In bacteria, transcription units can be insulated by placing a terminator in front of a promoter. In this way promoter leakage due to the read-through from an upstream gene or RNA polymerase unspecific binding to the DNA is, in principle, removed. Differently from bacterial terminators,...
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| Publicado no: | J Biol Eng |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5162094/ https://ncbi.nlm.nih.gov/pubmed/28018483 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13036-016-0040-5 |
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