Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applicati...

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Publicat a:Breast Cancer Res
Autors principals: Lloyd-Lewis, Bethan, Davis, Felicity M., Harris, Olivia B., Hitchcock, Jessica R., Lourenco, Filipe C., Pasche, Mathias, Watson, Christine J.
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2016
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5155399/
https://ncbi.nlm.nih.gov/pubmed/27964754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13058-016-0754-9
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