Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

BACKGROUND: High-resolution 3D imaging of intact tissue facilitates cellular and subcellular analyses of complex structures within their native environment. However, difficulties associated with immunolabelling and imaging fluorescent proteins deep within whole organs have restricted their applicati...

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Bibliografiske detaljer
Udgivet i:Breast Cancer Res
Main Authors: Lloyd-Lewis, Bethan, Davis, Felicity M., Harris, Olivia B., Hitchcock, Jessica R., Lourenco, Filipe C., Pasche, Mathias, Watson, Christine J.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2016
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5155399/
https://ncbi.nlm.nih.gov/pubmed/27964754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13058-016-0754-9
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