In vitro replication of cowpea mosaic virus RNA. II. Solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme.

A method for the solubilization of membrane-bound Cowpea mosaic virus RNA replicase has been developed by bypassing the use of detergents. Solubilization has been achieved by washing the 31,000 x g-pellet containing the bound replicase with a Mg2+-deficient buffer. This procedure had several advanta...

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Bibliografiske detaljer
Udgivet i:J Virol
Main Authors: Zabel, P, Jongen-Neven, I, Van Krammen, A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1976
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC515465/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1255853/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.17.3.679-685.1976
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