In vitro replication of cowpea mosaic virus RNA. II. Solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme.

A method for the solubilization of membrane-bound Cowpea mosaic virus RNA replicase has been developed by bypassing the use of detergents. Solubilization has been achieved by washing the 31,000 x g-pellet containing the bound replicase with a Mg2+-deficient buffer. This procedure had several advanta...

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Bibliographic Details
Main Authors: Zabel, P, Jongen-Neven, I, Van Krammen, A
Format: Artigo
Language:Inglês
Published: 1976
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC515465/
https://ncbi.nlm.nih.gov/pubmed/1255853
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