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In vitro replication of cowpea mosaic virus RNA. II. Solubilization of membrane-bound replicase and the partial purification of the solubilized enzyme.

A method for the solubilization of membrane-bound Cowpea mosaic virus RNA replicase has been developed by bypassing the use of detergents. Solubilization has been achieved by washing the 31,000 x g-pellet containing the bound replicase with a Mg2+-deficient buffer. This procedure had several advanta...

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Detaylı Bibliyografya
Asıl Yazarlar: Zabel, P, Jongen-Neven, I, Van Krammen, A
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1976
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC515465/
https://ncbi.nlm.nih.gov/pubmed/1255853
Etiketler: Etiketle
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