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Dynamics of CDKN1A in single cells defined by an endogenous fluorescent tagging toolkit

Observing the endogenous abundance, localization, and dynamics of proteins in mammalian cells is crucial to understanding their function and behavior. There is currently no systematic approach for fluorescent tagging of endogenous loci. Here we used Cas9 catalyzed DNA breaks, short homology arms, an...

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Publicat a:Cell Rep
Autors principals: Stewart-Ornstein, Jacob, Lahav, Galit
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5154611/
https://ncbi.nlm.nih.gov/pubmed/26876176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2016.01.045
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