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Dynamics of CDKN1A in single cells defined by an endogenous fluorescent tagging toolkit
Observing the endogenous abundance, localization, and dynamics of proteins in mammalian cells is crucial to understanding their function and behavior. There is currently no systematic approach for fluorescent tagging of endogenous loci. Here we used Cas9 catalyzed DNA breaks, short homology arms, an...
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| Publicado no: | Cell Rep |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5154611/ https://ncbi.nlm.nih.gov/pubmed/26876176 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2016.01.045 |
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