Role of Excess Inorganic Pyrophosphate in Primer-Extension Genotyping Assays

We have developed and genotyped >15,000 SNP assays by using a primer extension genotyping assay with fluorescence polarization (FP) detection. Although the 80% success rate of this assay is similar to those of other SNP genotyping assays, we wanted to determine the reasons for the failures and fi...

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Bibliografiske detaljer
Main Authors: Xiao, Ming, Phong, Angie, Lum, Kristen L., Greene, Richard A., Buzby, Philip R., Kwok, Pui-Yan
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2004
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC515321/
https://ncbi.nlm.nih.gov/pubmed/15310656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.2833204
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